Chromatography

Analyte

• In organic solvent soluble polymers
• Sample amount: ~ 100 mg
• Molar mass range: 10³ - 10⁷ g/mol

Equipment

• Eluent: organic solvents e.g. Tetrahydrofurane (THF), Chloroform (TCM), Dichloromethane (DCM), Hexafluoroisopropanol (HFIP), Toluol, Dimethylformamide (DMF), Dimethylacetamide (DMAc), Dimethylsulfoxide (DMSO), if necessary with salt additive e.g. NaNO₃, LiBr, CF₃COONa
• Detection: Refractive Index-Detector, UV-Detector, Viscometer, Multi Angle Laser Light Scattering Detector(MALLS), Evaporative Light Scattering Detector (ELSD), Refractive Index/Viscosity-Dual-Detektor
• Special feature: column tempering, Fractions collector for semi preparative use

Targets

• Conventional calibration with PS, PMMA, PEG, PEO, PLA and Pullulan
• Average molar masses (M_w , M_n); polydispersity index D
• Universal calibration by means of viscometry
• Intrinsic viscosity [ η ]; M_w; M_n; D
• Absolute molar mass determination by means of static light scattering
• Molar mass weight average M_W, average radius of gyration <R_gz>, M_n, D
• Additionally, in special cases an analysis of copolymer and structure composition can be performed.

Applications

• Reaction control of the synthesis of polyquinolines (PDF)
• Enzymatic modification of starch (PDF)
• Determination of molar masses of Lactic acid-based polymers and copolymers
• Evaluation of conversion during the synthesis of polymers
• Determination of molar masses of Polyethylene terephthalat and Polyacrylonitrile

Comparative and additional methods

• Membrane osmometry (M_n)
• Analytical ultracentrifugation (M_Z; D)
• Static light scattering (M_W; R_Z; A₂)
• Differential refraktometry (dn/dc)
• Dynamic light scattering (d_H)
• Vapor pressure osmometry (M_n)