Fluorescence spectrometry
Analyte
- Compact materials, films, solutions, dispersions, polymer layers on substrates
Equipment
- EquipmentSPEX (Jobin-Yvon) Fluorolog 122fluorescence spectral photometer LS50B (Perkin-Elmer)
- Wave length range: 200 – 800 nm (excitation), 200 – 900 nm (emission)
- the SPEX Fluorolog is able to record spectra from fluoresceine solutions with concentrations > 50 fmol/l
Analysis methods and response values
- Measuring of fluorescence spectra and measuring of fluorescence intensity depending on time ("time-drive")
Aim
- Determining position and intensity of fluorescence bands and their changes due to chemical and/or physical processes
- Investigations concerning the light-induced decomposition of substances
- Examination concerning the fluorescence behavior of additives (dyes) depending on the polymer matrix
- Measuring of fluorescence quenching in dependence on parameters like dye concentration, matrix material, production etc.
- charcterization of quantum dots
- labelling of functional groups for their determination