Molecular and colloid chemical characterization

Organic size exclusion chromatography (Organic GPC)


  • Polymers soluble in organic solvents
  • Sample size: ~ 100 mg
  • Molar mass range: 102 - 107 g/mol



  • Eluent: organic solvents such as tetrahydrofuran (THF), chloroform (TCM), dichloromethane (DCM), hexafluoroisopropanol (HFIP), toluene, dimethylformamide (DMF), dimethylacetamide (DMAc), dimethylsulfoxide (DMSO), if necessary with salt addition, e. g. NaNO3, LiBr, CF3COONa
  • Detectors: RI refractometer; UV detector; viscometer; multi-angle light scattering (MALLS), evaporative light scattering detector ELSD, RI / viscosity combination detector
  • Special features: column temperature control, fraction collector for semi-preparative use



  • Conventional calibration with PS, PMMA, PEG, PEO, PLA and Pullulan
  • Molar mass averages (MW, Mn), polydispersity index D
  • Universal calibration by viscometry
  • Intrinsic viscosity [ η ], MW, Mn, D
  • Absolute molar mass determination by means of static light scattering
  • Mass average MW, average gyration radius <Rgz>, Mn, D
  • Furthermore, copolymer or structural analysis can be performed in certain cases



  • Reaction monitoring of the synthesis of polyquinolines (see PDF)
  • Enzymatic modification of starch (see PDF)
  • Determination of the molar mass of polymers and copolymers based on lactic acid
  • Determination of the conversion rate in polymer synthesis
  • Determination of the molar mass of polyethylene terephthalates and polyacrylonitriles


Methods of comparison and supplementation

  • Membran osmometry (Mn)
  • Analytical ultracentrifugation (MZ; D)
  • Static light scattering (MW; RZ; A2)
  • Dynamic light scattering (dH)
  • Differential refractometry (dn/dc)
  • Vapor pressure osmometry (Mn)