Molecular and colloid chemical characterization

Dynamic light scattering (Dynamic LS)


  • Polymers, colloids and nanoparticles in solution (aqueous and organic solvent
  • Size range: 0.1 nm – 3 µm
  • Concentration range: 0.001 – 40 %



  • Delsa Nano +C (Beckman Coulter)
  • Delsa Nano Version 2.31


Evaluation methods and targets

  • Determination of the diffusion coefficient by dynamic light scattering
  • Mathematically determination of the particle size with different methods: Cumulant, Contin, Marquardt and NNLS
  • Ermittlung von Intensitäts-, Volumen- und Zahlenverteilungsfunktionen
  • The Calculation of Intensity-, Volume- and Numberdistributions


Application examples

  • Determination of hydrodynamic radius of polymers and polyelectrolytes with dependency on the solvent, electrolyte content and pH
  • Investigation of the formation of structures between two or more substances


Measuring principle

  • Investigations concerning the symplex formation between cationic starch and anionic polyelectrolytes (→ PDF)


Comparative and additional methods

  • Laser light diffraction
  • Analytical ultracentrifugation