Spectrometry

Analyte

• Liquid and solid samples
• Small, polar, ionic and thermally labile molecules as well as high molecular weight Biopolymers
• Assumption:
• Ionizable molecules – molecules with heteroatoms
• Soluble in suitable solvents

Equipment

• API 2000 Tripel-Quadrupol-Mass Spectrometer (AB Sciex)

Operating method

• Stand-alone: continuous sample feed - integrated injection pump
• Coupling with HPLC:
• Flow-injection (without HPLC-column)
• With former separation (HPLC, IC, GPC)
• Mass range 5 - 1800 m/z

Characteristic features

• Analytes are dissolved in solvents or solvent mixtures
• Soft ionization by spray technique
• Result: quasi-molecular ions as well as fragments
• High selectivity and detection sensitivity
• Quantitative analysis of the analytes possible - calibration necessary
• MS/MS delivers structural information

Applications

• Investigation of hydrolyzed starch- and cellulose derivates
• Identification and quantification of extractable polymer additive