Mass spectrometry (LC/MS/MS)
Analyte
- Liquid and solid samples
- Small, polar, ionic and thermally labile molecules as well as high molecular weight Biopolymers
- Assumption:
- Ionizable molecules – molecules with heteroatoms
- Soluble in suitable solvents
Equipment
- API 2000 Tripel-Quadrupol-Mass Spectrometer (AB Sciex)
Operating method
- Stand-alone: continuous sample feed - integrated injection pump
- Coupling with HPLC:
- Flow-injection (without HPLC-column)
- With former separation (HPLC, IC, GPC)
- Mass range 5 - 1800 m/z
Characteristic features
- Analytes are dissolved in solvents or solvent mixtures
- Soft ionization by spray technique
- Result: quasi-molecular ions as well as fragments
- High selectivity and detection sensitivity
- Quantitative analysis of the analytes possible - calibration necessary
- MS/MS delivers structural information
Applications
- Investigation of hydrolyzed starch- and cellulose derivates
- Identification and quantification of extractable polymer additive