Organic size exclusion chromatography (SEC)


  • In organic solvent soluble polymers
  • Sample amount: ~ 100 mg
  • Molar mass range: 103 - 107 g/mol



  • Eluent: organic solvents e.g. Tetrahydrofurane (THF), Chloroform (TCM), Dichloromethane (DCM), Hexafluoroisopropanol (HFIP), Toluol, Dimethylformamide (DMF), Dimethylacetamide (DMAc), Dimethylsulfoxide (DMSO), if necessary with salt additive e.g. NaNO3, LiBr, CF3COONa
  • Detection: Refractive Index-Detector, UV-Detector, Viscometer, Multi Angle Laser Light Scattering Detector(MALLS), Evaporative Light Scattering Detector (ELSD), Refractive Index/Viscosity-Dual-Detektor
  • Special feature: column tempering, Fractions collector for semi preparative use



  • Conventional calibration with PS, PMMA, PEG, PEO, PLA and Pullulan
  • Average molar masses (Mw , Mn); polydispersity index D
  • Universal calibration by means of viscometry
  • Intrinsic viscosity [ η ]; Mw; Mn; D
  • Absolute molar mass determination by means of static light scattering
  • Molar mass weight average MW, average radius of gyration <Rgz>, Mn, D
  • Additionally, in special cases an analysis of copolymer and structure composition can be performed.



  • Reaction control of the synthesis of polyquinolines (PDF)
  • Enzymatic modification of starch (PDF)
  • Determination of molar masses of Lactic acid-based polymers and copolymers
  • Evaluation of conversion during the synthesis of polymers
  • Determination of molar masses of Polyethylene terephthalat and Polyacrylonitrile


Comparative and additional methods

  • Membrane osmometry (Mn)
  • Analytical ultracentrifugation (MZ; D)
  • Static light scattering (MW; RZ; A2)
  • Differential refraktometry (dn/dc)
  • Dynamic light scattering (dH)
  • Vapor pressure osmometry (Mn)