Organic size exclusion chromatography (SEC)
Analyte
- In organic solvent soluble polymers
- Sample amount: ~ 100 mg
- Molar mass range: 103 - 107 g/mol
Equipment
- Eluent: organic solvents e.g. Tetrahydrofurane (THF), Chloroform (TCM), Dichloromethane (DCM), Hexafluoroisopropanol (HFIP), Toluol, Dimethylformamide (DMF), Dimethylacetamide (DMAc), Dimethylsulfoxide (DMSO), if necessary with salt additive e.g. NaNO3, LiBr, CF3COONa
- Detection: Refractive Index-Detector, UV-Detector, Viscometer, Multi Angle Laser Light Scattering Detector(MALLS), Evaporative Light Scattering Detector (ELSD), Refractive Index/Viscosity-Dual-Detektor
- Special feature: column tempering, Fractions collector for semi preparative use
Targets
- Conventional calibration with PS, PMMA, PEG, PEO, PLA and Pullulan
- Average molar masses (Mw , Mn); polydispersity index D
- Universal calibration by means of viscometry
- Intrinsic viscosity [ η ]; Mw; Mn; D
- Absolute molar mass determination by means of static light scattering
- Molar mass weight average MW, average radius of gyration <Rgz>, Mn, D
- Additionally, in special cases an analysis of copolymer and structure composition can be performed.
Applications
- Reaction control of the synthesis of polyquinolines (PDF)
- Enzymatic modification of starch (PDF)
- Determination of molar masses of Lactic acid-based polymers and copolymers
- Evaluation of conversion during the synthesis of polymers
- Determination of molar masses of Polyethylene terephthalat and Polyacrylonitrile
Comparative and additional methods
- Membrane osmometry (Mn)
- Analytical ultracentrifugation (MZ; D)
- Static light scattering (MW; RZ; A2)
- Differential refraktometry (dn/dc)
- Dynamic light scattering (dH)
- Vapor pressure osmometry (Mn)