Raman spectroscopy


  • Solid and liquid polymers; composites, materials, organic and anorganic low-molecular substances, thin layers, fibres, solution
  • Sample amount: ca. 1 mg



Bruker RFS 100/S FT Ramanspektrometer with coppled RamanScope III microscope and light-conducting fiber with head RAMPROBE

  • Spectral range 3600 – 50 cm-1 (Stokes) and 100 – 2000 cm-1 (anti-Stokes)
  • Fluorescence-free Raman spectra by excitation at 1064 nm (Nd:YAG-Laser)
  • liquid nitrogen cooled, Ge detector
  • Objective: 10x, NA = 0,25; WD = 6,5 mm und 100x, NA = 1,25; WD = 0,15 (microscope)
  • motorised sample stage, intercept length 1 µm (microscope)
  • Immersion tube: RT201-25, maximum operating temperature: 180 °C, maximum pressure: 2 bar
  • Variable temperature cell


Evaluation methods and targets

Fluorescence-free Raman spectra

  • Wave number
  • Raman intensity



  • Idendification of functional groups
  • Quantitative determination of degrees of substitution of polysaccharides
  • Characterisation of cellulose structures
  • Investigation of cellulose modification in solutions or melts
  • Reaction control by online measurement in the reactor
  • FT Raman microscopy at plant fibres


Application examples

  • Determination of degrees of substitution of carboxymethyl starch, carboxymethyl cellulose and starch acetate