Continuous HPLC (CHPLC)

Modus Operandi

The functional principle of the developed continuous HPLC is based on an interference of the flow of the mobile phase with the rotation of of the stationary phase. This was achieved through the use of 16 identical columns interconnected by a new multifunctional valve. Through this, the temporally offset elution of the different components of the sample solution in a single immobile stationary phase (single column of standard HPLC) was transposed into a spacially offset separation from a rotating stationary phase.This kind of column circuitry enables both continuous injection of the mixture to be separated and continuous elution of its separated components. The separation characterictics of the single columns remain the same. Furthermore the variable dimensioning of the used stationary phase and the different interconnection possibilities of the system components provide both optimal customization to requirements of semi-preparative applications and continuous liquid-chromatographic analysis of reaction kinetics.


Application Range

  • continuous analysis of reaction kinetics
  • process control
  • extraction of pure substances in gram scale



This work was funded by the BMBF, Dept. 523 under the support code 01 VQ 943A and took place in co-operation with Dr. Ing. Herbert Knauer GmbH and Ruhr-University Bochum, Chair of Analytical Chemistry, RG Prof. H.-J. Götze.



J. Bauer, H.-J. Götze, T. Kiffmeyer, M. Jursch: Kontinuierliche ortsaufgelöste HPLC auf Basis eines Multifunktionsventils - Prinzip und Anwendungen, GIT 41, p. 6-9 (1997)