Chromatography

Analyte

Solid und liquid sample mixtures, soluble in a solvent or solvent mixture

Preferred substances:

• Difficult-to-volatilize
• High polarity and ionic
• Thermolabile and easily decomposable
• With high molecular mass

Equipment

2 analytical HPLC-plants Series 200, Perkin Elmer Co.

• Quaternary Pump
• Automatic sampler
• Detector: LC/MS/MS, Diode-Array-Detector DAD, UV/VIS-Detector, Refractive Index-Detector RI
• Column oven
• Fraction collector for semipreparative use
• Different HPLC columns for reversed phase and normal phase chromatography

Targets

• Separation of sample mixtures
• Qualitative and quantitative detection of single components
• Calibration with reference substances as internal and external standards
• Identification of substances by comparison, UV-Spectra (DAD) or mass spectra (LC/MS/MS)

Applications

• Purity and product control, quality assurance
• Process control and process development
• By-product analysis
• Characterization of polymers
• Purification und separation of single components
• Determination of carbohydrates