Chromatography

Analyte

• Polyelectrolytes as well as nonionic water soluble polymers and polysaccharides
• Sample mass: ~ 100 mg
• Molar mass range: 10³ - 10⁸ g/mol

Equipment

• Eluent: aqueous salt solution (if required with organic co-eluent) e.g. 0.2 M Na₂SO₄+1% HAc
• Detection: refractometer, UV-detector, viscometer, multi angle laser light scattering (MALLS)
• Special feature: column tempering, Fraction collector
  

Targets

• Conventional calibration with Dextran, Pullulan, polyvinylpyridinium and polystyrene sulfonic acid
• average molar masses (M_w , M_n); polydispersity index D
• Universal calibration by means of viscometry
• intrinsic viscosity [ η ]; M_w; M_n; D
• Absolute molar mass determination by means of static light scattering
• molar mass weight average M_W, average radius of gyration <R_gz>, M_n, D
• Additionally, in special cases an analysis of copolymer and structure composition can be performed.

Application

• Graft copolymerization of cationic monomers on starch (PDF)
  
• Enzymatic synthesis of high molecular Inulin (PDF)
  
• Derivatization of starch (PDF)
  

Comparative and Complementary Methods

• Membrane osmometry (M_n)
• Analytical ultracentrifugation (M_Z; D)
  
• Static light scattering (M_W; R_Z; A₂)
• Dynamic light scattering (d_H)
• Differential refractometry (dn/dc)
• Vapor-pressure osmometry (M_n)