Molecular and colloid chemical characterization

Analyte

• Polyelectrolytes as well as non-ionic water-soluble polymers and polysaccharides
• Sample quantity: approx. 10 mg
• Molar mass range: 10²–10⁸ g/mol

Equipment

• Eluents: aqueous salt solutions, e.g. with organic coeluents, e.g. 0.2 M Na₂SO₄+1% HAc
• Detectors: RI refractometer, UV detector, viscometer, multi-angle light scattering (MALLS)
• Special features: columetric temperature control, fragment collector
  

Target

• Conventional calibration with dextran, pullulan, polyvinylpyridine and polystyrene sulfonic acid
  • Molar mass averages (M_W, M_n), polydispersity index D
• On request, preparation and use of further standards via controlled radical polymerization
• Universal calibration by viscometry
  • Intrinsic viscosity [ η ], M_W, M_n, D
• Absolute molar mass analysis by means of static light scattering
  • Mass average M_W, average gyration radius <R_gz>, M_n, D

• On demand, combination with further methods of molecular analysis, e. g. ¹H-NMR and IR spectroscopy, e. g. for structure determination or for determination of the composition of copolymers

Application

• Graft copolymerization of cationic monomers onto starch (see PDF)
• Enzymatic synthesis of high molecular weight inulin (see PDF)
• Derivatization of starch (see PDF)

Methods of comparison and supplementation

• Membrane osmometry (M_n)
• Analytical ultracentrifugation (M_Z; D)
  
• Static light scattering (M_W; R_Z; A₂)
• Dynamic light scattering (d_H)
• Differential refractometry (dn/dc)
• Vapor pressure osmometry (M_n)